Toward High-Throughput Analysis of Aroma Compounds Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry: Screening of Key Food Odorants in Various Foods

Markus Bösl; Andreas Dunkel; Daniela Hartl; Anja Dollinger; Andrea Spaccasassi; Timo D. Stark; Corinna Dawid; Thomas F. Hofmann

doi:10.1021/acs.jafc.3c00935

Toward High-Throughput Analysis of Aroma Compounds Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry: Screening of Key Food Odorants in Various Foods

Markus Bösl (First Author), Andreas Dunkel (Co-Author), Daniela Hartl (Co-Author), Anja Dollinger (Co-Author), Andrea Spaccasassi (Co-Author), Timo D. Stark (Co-Author), Corinna Dawid^* (Co-Author), Thomas F. Hofmann^* (Last Author)

^*Corresponding author for this work

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Recent studies show the immense capacities of the unified quantitation of aroma and taste compounds using liquid chromatography-mass spectrometry (LC-MS). The goal of this study was to highlight the broad application of this unified method. Thus, a stable isotope dilution analysis quantification method of the most important key food odorants in various food categories by LC-MS was developed. Using the well-known derivatization agent 3-nitrophenylhydrazine for carbonyl derivatization and a newly developed approach for alcohol and thiol derivatization, a method for the quantitation of 20 key food odorants was established. Intraday precision was determined to be ≤26%, and interday precision was between 24 and 31%. Limits of quantitation were determined between 0.014 and 283 μg/kg. The work shows that a wide array of aroma compounds can be analyzed accurately by LC-MS.

Original language	English
Pages (from-to)	8622-8632
Number of pages	11
Journal	Journal of Agricultural and Food Chemistry
Volume	71
Issue number	22
DOIs	https://doi.org/10.1021/acs.jafc.3c00935
State	Published - 7 Jun 2023

Keywords

3-nitrophenylhydrazine
LC-MS
aroma analysis
flavor analysis
generalists
stable isotope dilution assay

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10.1021/acs.jafc.3c00935

Cite this

Bösl, M., Dunkel, A., Hartl, D., Dollinger, A., Spaccasassi, A., Stark, T. D., Dawid, C., & Hofmann, T. F. (2023). Toward High-Throughput Analysis of Aroma Compounds Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry: Screening of Key Food Odorants in Various Foods. Journal of Agricultural and Food Chemistry, 71(22), 8622-8632. https://doi.org/10.1021/acs.jafc.3c00935

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title = "Toward High-Throughput Analysis of Aroma Compounds Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry: Screening of Key Food Odorants in Various Foods",

abstract = "Recent studies show the immense capacities of the unified quantitation of aroma and taste compounds using liquid chromatography-mass spectrometry (LC-MS). The goal of this study was to highlight the broad application of this unified method. Thus, a stable isotope dilution analysis quantification method of the most important key food odorants in various food categories by LC-MS was developed. Using the well-known derivatization agent 3-nitrophenylhydrazine for carbonyl derivatization and a newly developed approach for alcohol and thiol derivatization, a method for the quantitation of 20 key food odorants was established. Intraday precision was determined to be ≤26\%, and interday precision was between 24 and 31\%. Limits of quantitation were determined between 0.014 and 283 μg/kg. The work shows that a wide array of aroma compounds can be analyzed accurately by LC-MS.",

keywords = "3-nitrophenylhydrazine, LC-MS, aroma analysis, flavor analysis, generalists, stable isotope dilution assay",

author = "Markus B{\"o}sl and Andreas Dunkel and Daniela Hartl and Anja Dollinger and Andrea Spaccasassi and Stark, \{Timo D.\} and Corinna Dawid and Hofmann, \{Thomas F.\}",

year = "2023",

month = jun,

day = "7",

doi = "10.1021/acs.jafc.3c00935",

language = "English",

volume = "71",

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Bösl, M, Dunkel, A, Hartl, D, Dollinger, A, Spaccasassi, A, Stark, TD, Dawid, C & Hofmann, TF 2023, 'Toward High-Throughput Analysis of Aroma Compounds Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry: Screening of Key Food Odorants in Various Foods', Journal of Agricultural and Food Chemistry, vol. 71, no. 22, pp. 8622-8632. https://doi.org/10.1021/acs.jafc.3c00935

Toward High-Throughput Analysis of Aroma Compounds Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry: Screening of Key Food Odorants in Various Foods. / Bösl, Markus (First Author); Dunkel, Andreas (Co-Author); Hartl, Daniela (Co-Author) et al.
In: Journal of Agricultural and Food Chemistry, Vol. 71, No. 22, 07.06.2023, p. 8622-8632.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Toward High-Throughput Analysis of Aroma Compounds Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry

T2 - Screening of Key Food Odorants in Various Foods

AU - Bösl, Markus

AU - Dunkel, Andreas

AU - Hartl, Daniela

AU - Dollinger, Anja

AU - Spaccasassi, Andrea

AU - Stark, Timo D.

AU - Dawid, Corinna

AU - Hofmann, Thomas F.

PY - 2023/6/7

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AB - Recent studies show the immense capacities of the unified quantitation of aroma and taste compounds using liquid chromatography-mass spectrometry (LC-MS). The goal of this study was to highlight the broad application of this unified method. Thus, a stable isotope dilution analysis quantification method of the most important key food odorants in various food categories by LC-MS was developed. Using the well-known derivatization agent 3-nitrophenylhydrazine for carbonyl derivatization and a newly developed approach for alcohol and thiol derivatization, a method for the quantitation of 20 key food odorants was established. Intraday precision was determined to be ≤26%, and interday precision was between 24 and 31%. Limits of quantitation were determined between 0.014 and 283 μg/kg. The work shows that a wide array of aroma compounds can be analyzed accurately by LC-MS.

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KW - flavor analysis

KW - generalists

KW - stable isotope dilution assay

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Toward High-Throughput Analysis of Aroma Compounds Using Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry: Screening of Key Food Odorants in Various Foods

Abstract

Keywords

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