Syntheses of labeled vitamers of folic acid to be used as internal standards in stable isotope dilution assays

Achim Freisleben; Peter Schieberle; Michael Rychlik

doi:10.1021/jf025571k

Syntheses of labeled vitamers of folic acid to be used as internal standards in stable isotope dilution assays

Achim Freisleben, Peter Schieberle, Michael Rychlik^*

^*Corresponding author for this work

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

[²H₄]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin, Using [²H₄]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.

Original language	English
Pages (from-to)	4760-4768
Number of pages	9
Journal	Journal of Agricultural and Food Chemistry
Volume	50
Issue number	17
DOIs	https://doi.org/10.1021/jf025571k
State	Published - 14 Aug 2002
Externally published	Yes

Keywords

Electrospray mass spectrometry
Folates
Labeled folic acid
Stable isotope dilution assay

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10.1021/jf025571k

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abstract = "[2H4]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin, Using [2H4]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.",

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author = "Achim Freisleben and Peter Schieberle and Michael Rychlik",

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doi = "10.1021/jf025571k",

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AU - Freisleben, Achim

AU - Schieberle, Peter

AU - Rychlik, Michael

PY - 2002/8/14

Y1 - 2002/8/14

N2 - [2H4]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin, Using [2H4]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.

AB - [2H4]Folic acid was synthesized by deuterating p-aminobenzoic acid, which was then coupled to glutamic acid and 6-formylpterin, Using [2H4]folic acid as starting component enabled the preparation of labeled vitamers tetrahydrofolate, 5-formyltetrahydrofolate, 5-methyltetrahydrofolate, and 10-formylfolate which were characterized by electrospray mass spectrometry and collision-induced dissociation. The mass spectrometric studies confirmed that the compounds could be used as internal standards in stable isotope dilution assays.

KW - Electrospray mass spectrometry

KW - Folates

KW - Labeled folic acid

KW - Stable isotope dilution assay

UR - https://www.scopus.com/pages/publications/0037077404

U2 - 10.1021/jf025571k

DO - 10.1021/jf025571k

M3 - Article

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AN - SCOPUS:0037077404

SN - 0021-8561

VL - 50

SP - 4760

EP - 4768

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 17

ER -

Syntheses of labeled vitamers of folic acid to be used as internal standards in stable isotope dilution assays

Abstract

Keywords

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