Sodium-ion binding to DNA: Detection by ultrafast time-resolved stokes-shift spectroscopy

Latha A. Gearheart; Mark M. Somoza; W. Evan Rivers; Catherine J. Murphy; Robert S. Coleman; Mark A. Berg

doi:10.1021/ja0363617

Sodium-ion binding to DNA: Detection by ultrafast time-resolved stokes-shift spectroscopy

Latha A. Gearheart (First Author), Mark M. Somoza (Co-Author), W. Evan Rivers (Co-Author), Catherine J. Murphy (Co-Author), Robert S. Coleman (Co-Author), Mark A. Berg^* (Last Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

The nature of the counterion is shown to have a pronounced effect on the apparent dynamic polarity in the interior of DNA. Time-resolved Stokes-shift measurements in the 80 ps to 40 ns time range were made on a polarity-sensitive fluorophore (coumarin 102) that replaces a base pair in an oligonucleotide. With sodium counterions, the emission spectrum narrows with time, whereas with tetrabutylammonium counterions, it does not. Our interpretation is that a subpopulation of helices have sodium cations bound in a fashion that slows the normal dynamics.

Original language	English
Pages (from-to)	11812-11813
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	125
Issue number	39
DOIs	https://doi.org/10.1021/ja0363617
State	Published - 1 Oct 2003
Externally published	Yes

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title = "Sodium-ion binding to DNA: Detection by ultrafast time-resolved stokes-shift spectroscopy",

abstract = "The nature of the counterion is shown to have a pronounced effect on the apparent dynamic polarity in the interior of DNA. Time-resolved Stokes-shift measurements in the 80 ps to 40 ns time range were made on a polarity-sensitive fluorophore (coumarin 102) that replaces a base pair in an oligonucleotide. With sodium counterions, the emission spectrum narrows with time, whereas with tetrabutylammonium counterions, it does not. Our interpretation is that a subpopulation of helices have sodium cations bound in a fashion that slows the normal dynamics.",

author = "Gearheart, \{Latha A.\} and Somoza, \{Mark M.\} and Rivers, \{W. Evan\} and Murphy, \{Catherine J.\} and Coleman, \{Robert S.\} and Berg, \{Mark A.\}",

year = "2003",

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T1 - Sodium-ion binding to DNA

T2 - Detection by ultrafast time-resolved stokes-shift spectroscopy

AU - Gearheart, Latha A.

AU - Somoza, Mark M.

AU - Rivers, W. Evan

AU - Murphy, Catherine J.

AU - Coleman, Robert S.

AU - Berg, Mark A.

PY - 2003/10/1

Y1 - 2003/10/1

N2 - The nature of the counterion is shown to have a pronounced effect on the apparent dynamic polarity in the interior of DNA. Time-resolved Stokes-shift measurements in the 80 ps to 40 ns time range were made on a polarity-sensitive fluorophore (coumarin 102) that replaces a base pair in an oligonucleotide. With sodium counterions, the emission spectrum narrows with time, whereas with tetrabutylammonium counterions, it does not. Our interpretation is that a subpopulation of helices have sodium cations bound in a fashion that slows the normal dynamics.

AB - The nature of the counterion is shown to have a pronounced effect on the apparent dynamic polarity in the interior of DNA. Time-resolved Stokes-shift measurements in the 80 ps to 40 ns time range were made on a polarity-sensitive fluorophore (coumarin 102) that replaces a base pair in an oligonucleotide. With sodium counterions, the emission spectrum narrows with time, whereas with tetrabutylammonium counterions, it does not. Our interpretation is that a subpopulation of helices have sodium cations bound in a fashion that slows the normal dynamics.

UR - https://www.scopus.com/pages/publications/0141732256

U2 - 10.1021/ja0363617

DO - 10.1021/ja0363617

M3 - Article

C2 - 14505391

AN - SCOPUS:0141732256

SN - 0002-7863

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EP - 11813

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 39

ER -

Sodium-ion binding to DNA: Detection by ultrafast time-resolved stokes-shift spectroscopy

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