In-situ chemical synthesis of rU-DNA chimeras on chips and enzymatic recognition

Jeremy G. Lackey; Mark M. Somoza; Debbie Mitra; Franco Cerrina; Masad J. Damha

In-situ chemical synthesis of rU-DNA chimeras on chips and enzymatic recognition

Jeremy G. Lackey (First Author), Mark M. Somoza (Co-Author), Debbie Mitra (Co-Author), Franco Cerrina (Co-Author), Masad J. Damha^* (Last Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

We describe the light-directed in sitv synthesis of rU-DNA chimeric oligonucleotides on microarrays. An RNase A assay was performed as "proof-of-principle" to demonstrate the value of such microarrays for studying enzyme kinetics and specificity on RNA based libraries.

Original language	English
Pages (from-to)	30-33
Number of pages	4
Journal	Chimica Oggi
Volume	27
Issue number	6
State	Published - Nov 2009
Externally published	Yes

Cite this

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title = "In-situ chemical synthesis of rU-DNA chimeras on chips and enzymatic recognition",

abstract = "We describe the light-directed in sitv synthesis of rU-DNA chimeric oligonucleotides on microarrays. An RNase A assay was performed as {"}proof-of-principle{"} to demonstrate the value of such microarrays for studying enzyme kinetics and specificity on RNA based libraries.",

author = "Lackey, \{Jeremy G.\} and Somoza, \{Mark M.\} and Debbie Mitra and Franco Cerrina and Damha, \{Masad J.\}",

year = "2009",

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language = "English",

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journal = "Chimica Oggi",

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T1 - In-situ chemical synthesis of rU-DNA chimeras on chips and enzymatic recognition

AU - Lackey, Jeremy G.

AU - Somoza, Mark M.

AU - Mitra, Debbie

AU - Cerrina, Franco

AU - Damha, Masad J.

PY - 2009/11

Y1 - 2009/11

N2 - We describe the light-directed in sitv synthesis of rU-DNA chimeric oligonucleotides on microarrays. An RNase A assay was performed as "proof-of-principle" to demonstrate the value of such microarrays for studying enzyme kinetics and specificity on RNA based libraries.

AB - We describe the light-directed in sitv synthesis of rU-DNA chimeric oligonucleotides on microarrays. An RNase A assay was performed as "proof-of-principle" to demonstrate the value of such microarrays for studying enzyme kinetics and specificity on RNA based libraries.

UR - https://www.scopus.com/pages/publications/76149132678

M3 - Article

AN - SCOPUS:76149132678

SN - 0392-839X

VL - 27

SP - 30

EP - 33

JO - Chimica Oggi

JF - Chimica Oggi

IS - 6

ER -

In-situ chemical synthesis of rU-DNA chimeras on chips and enzymatic recognition

Abstract

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