Impact of lactisole on the time-intensity profile of selected sweeteners in dependence of the binding site

Corinna M. Deck; Maik Behrens; Martin Wendelin; Jakob P. Ley; Gerhard E. Krammer; Barbara Lieder

doi:10.1016/j.fochx.2022.100446

Impact of lactisole on the time-intensity profile of selected sweeteners in dependence of the binding site

Corinna M. Deck (First Author), Maik Behrens (Co-Author), Martin Wendelin (Co-Author), Jakob P. Ley (Co-Author), Gerhard E. Krammer (Co-Author), Barbara Lieder^* (Last Author)

^*Corresponding author for this work

Taste & Odor Systems Reception

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Currently, there is limited insight into the influence of the different binding sites of agonists and antagonists of the sweet taste receptor TAS1R2/TAS1R3 on temporal sensory properties of sweet tasting compounds. We investigated whether the binding site and a competitive or allosteric inhibition of TAS1R2/TAS1R3 influence the time-dependent sensory perception and in vitro TAS1R2/TAS1R3-activation profiles. We compared time-intensity ratings of cyclamate, NHDC, acesulfame K, and aspartame with and without lactisole with the corresponding TAS1R2/TAS1R3-activation in transfected HEK293 cells. In combination with lactisole, cyclamate and NHDC demonstrated a shift of the dose–response curve corresponding to a competitive inhibition by lactisole in the sensory and the cell experiments. Allosteric inhibition by lactisole for aspartame and acesulfame K was seen in the cell experiments, but not the sensory ratings. In conclusion, the data do not support a major impact of the binding site on the time-intensity profile of the tested sweeteners.

Original language	English
Article number	100446
Journal	Food Chemistry: X
Volume	15
DOIs	https://doi.org/10.1016/j.fochx.2022.100446
State	Published - 30 Oct 2022

Keywords

Acesulfame K
Aspartame
Cyclamate
NHDC
Sweet taste receptor
Time-intensity

Access to Document

10.1016/j.fochx.2022.100446

Cite this

@article{eb1f709109a74e8caa20fd753a3acf60,

title = "Impact of lactisole on the time-intensity profile of selected sweeteners in dependence of the binding site",

abstract = "Currently, there is limited insight into the influence of the different binding sites of agonists and antagonists of the sweet taste receptor TAS1R2/TAS1R3 on temporal sensory properties of sweet tasting compounds. We investigated whether the binding site and a competitive or allosteric inhibition of TAS1R2/TAS1R3 influence the time-dependent sensory perception and in vitro TAS1R2/TAS1R3-activation profiles. We compared time-intensity ratings of cyclamate, NHDC, acesulfame K, and aspartame with and without lactisole with the corresponding TAS1R2/TAS1R3-activation in transfected HEK293 cells. In combination with lactisole, cyclamate and NHDC demonstrated a shift of the dose–response curve corresponding to a competitive inhibition by lactisole in the sensory and the cell experiments. Allosteric inhibition by lactisole for aspartame and acesulfame K was seen in the cell experiments, but not the sensory ratings. In conclusion, the data do not support a major impact of the binding site on the time-intensity profile of the tested sweeteners.",

keywords = "Acesulfame K, Aspartame, Cyclamate, NHDC, Sweet taste receptor, Time-intensity",

author = "Deck, \{Corinna M.\} and Maik Behrens and Martin Wendelin and Ley, \{Jakob P.\} and Krammer, \{Gerhard E.\} and Barbara Lieder",

note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",

year = "2022",

month = oct,

day = "30",

doi = "10.1016/j.fochx.2022.100446",

language = "English",

volume = "15",

journal = "Food Chemistry: X",

issn = "2590-1575",

}

TY - JOUR

T1 - Impact of lactisole on the time-intensity profile of selected sweeteners in dependence of the binding site

AU - Deck, Corinna M.

AU - Behrens, Maik

AU - Wendelin, Martin

AU - Ley, Jakob P.

AU - Krammer, Gerhard E.

AU - Lieder, Barbara

PY - 2022/10/30

Y1 - 2022/10/30

N2 - Currently, there is limited insight into the influence of the different binding sites of agonists and antagonists of the sweet taste receptor TAS1R2/TAS1R3 on temporal sensory properties of sweet tasting compounds. We investigated whether the binding site and a competitive or allosteric inhibition of TAS1R2/TAS1R3 influence the time-dependent sensory perception and in vitro TAS1R2/TAS1R3-activation profiles. We compared time-intensity ratings of cyclamate, NHDC, acesulfame K, and aspartame with and without lactisole with the corresponding TAS1R2/TAS1R3-activation in transfected HEK293 cells. In combination with lactisole, cyclamate and NHDC demonstrated a shift of the dose–response curve corresponding to a competitive inhibition by lactisole in the sensory and the cell experiments. Allosteric inhibition by lactisole for aspartame and acesulfame K was seen in the cell experiments, but not the sensory ratings. In conclusion, the data do not support a major impact of the binding site on the time-intensity profile of the tested sweeteners.

AB - Currently, there is limited insight into the influence of the different binding sites of agonists and antagonists of the sweet taste receptor TAS1R2/TAS1R3 on temporal sensory properties of sweet tasting compounds. We investigated whether the binding site and a competitive or allosteric inhibition of TAS1R2/TAS1R3 influence the time-dependent sensory perception and in vitro TAS1R2/TAS1R3-activation profiles. We compared time-intensity ratings of cyclamate, NHDC, acesulfame K, and aspartame with and without lactisole with the corresponding TAS1R2/TAS1R3-activation in transfected HEK293 cells. In combination with lactisole, cyclamate and NHDC demonstrated a shift of the dose–response curve corresponding to a competitive inhibition by lactisole in the sensory and the cell experiments. Allosteric inhibition by lactisole for aspartame and acesulfame K was seen in the cell experiments, but not the sensory ratings. In conclusion, the data do not support a major impact of the binding site on the time-intensity profile of the tested sweeteners.

KW - Acesulfame K

KW - Aspartame

KW - Cyclamate

KW - NHDC

KW - Sweet taste receptor

KW - Time-intensity

UR - https://www.scopus.com/pages/publications/85138142841

U2 - 10.1016/j.fochx.2022.100446

DO - 10.1016/j.fochx.2022.100446

M3 - Article

AN - SCOPUS:85138142841

SN - 2590-1575

VL - 15

JO - Food Chemistry: X

JF - Food Chemistry: X

M1 - 100446

ER -

Impact of lactisole on the time-intensity profile of selected sweeteners in dependence of the binding site

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this