Effect of coffee combining green coffee bean constituents with typical roasting products on the Nrf2/ARE pathway in vitro and in vivo

Nadine Volz; Ute Boettler; Swantje Winkler; Nicole Teller; Christoph Schwarz; Tamara Bakuradze; Gerhard Eisenbrand; Larissa Haupt; Lyn R. Griffiths; Herbert Stiebitz; Gerhard Bytof; Ingo Lantz; Roman Lang; Thomas Hofmann; Veronika Somoza; Doris Marko

doi:10.1021/jf302258u

Effect of coffee combining green coffee bean constituents with typical roasting products on the Nrf2/ARE pathway in vitro and in vivo

Nadine Volz (First Author), Ute Boettler (Co-Author), Swantje Winkler (Co-Author), Nicole Teller (Co-Author), Christoph Schwarz (Co-Author), Tamara Bakuradze (Co-Author), Gerhard Eisenbrand (Co-Author), Larissa Haupt (Co-Author), Lyn R. Griffiths (Co-Author), Herbert Stiebitz (Co-Author), Gerhard Bytof (Co-Author), Ingo Lantz (Co-Author), Roman Lang (Co-Author), Thomas Hofmann (Co-Author), Veronika Somoza (Co-Author), Doris Marko^* (Last Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

This study investigated Nrf2-activating properties of a coffee blend combining raw coffee bean constituents with 5-O-caffeoylquinic acid (CGA) as a lead component with typical roasting products such as N-methylpyridinium (NMP). In cell culture (HT29) the respective coffee extract (CN-CE) increased nuclear Nrf2 translocation and enhanced the transcription of ARE-dependent genes as exemplified for NAD(P)H:quinone oxidoreductase and glutathione-S-transferase (GST)A1, reflected in the protein level by an increase in GST enzyme activity. In a pilot human intervention study (29 healthy volunteers), daily consumption of 750 mL of CN-coffee for 4 weeks increased Nrf2 transcription in peripheral blood lymphocytes on average. However, the transcriptional response pattern of Nrf2/ARE-dependent genes showed substantial interindividual variations. The presence of SNPs in the Nrf2-promoter, reported recently, as well as the detection of GSTT1*0 (null) genotypes in the study collective strengthens the hypothesis that coffee acts as a modulator of Nrf2-dependent gene response in humans, but genetic polymorphisms play an important role in the individual response pattern.

Original language	English
Pages (from-to)	9631-9641
Number of pages	11
Journal	Journal of Agricultural and Food Chemistry
Volume	60
Issue number	38
DOIs	https://doi.org/10.1021/jf302258u
State	Published - 26 Sep 2012
Externally published	Yes

Keywords

N-methylpyridinium
Nrf2
chlorogenic acid
human intervention study
phase II enzymes

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10.1021/jf302258u

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Volz, N., Boettler, U., Winkler, S., Teller, N., Schwarz, C., Bakuradze, T., Eisenbrand, G., Haupt, L., Griffiths, L. R., Stiebitz, H., Bytof, G., Lantz, I., Lang, R., Hofmann, T., Somoza, V., & Marko, D. (2012). Effect of coffee combining green coffee bean constituents with typical roasting products on the Nrf2/ARE pathway in vitro and in vivo. Journal of Agricultural and Food Chemistry, 60(38), 9631-9641. https://doi.org/10.1021/jf302258u

@article{887c3db239fc4e058f7ecd30e3a48cad,

title = "Effect of coffee combining green coffee bean constituents with typical roasting products on the Nrf2/ARE pathway in vitro and in vivo",

abstract = "This study investigated Nrf2-activating properties of a coffee blend combining raw coffee bean constituents with 5-O-caffeoylquinic acid (CGA) as a lead component with typical roasting products such as N-methylpyridinium (NMP). In cell culture (HT29) the respective coffee extract (CN-CE) increased nuclear Nrf2 translocation and enhanced the transcription of ARE-dependent genes as exemplified for NAD(P)H:quinone oxidoreductase and glutathione-S-transferase (GST)A1, reflected in the protein level by an increase in GST enzyme activity. In a pilot human intervention study (29 healthy volunteers), daily consumption of 750 mL of CN-coffee for 4 weeks increased Nrf2 transcription in peripheral blood lymphocytes on average. However, the transcriptional response pattern of Nrf2/ARE-dependent genes showed substantial interindividual variations. The presence of SNPs in the Nrf2-promoter, reported recently, as well as the detection of GSTT1*0 (null) genotypes in the study collective strengthens the hypothesis that coffee acts as a modulator of Nrf2-dependent gene response in humans, but genetic polymorphisms play an important role in the individual response pattern.",

keywords = "N-methylpyridinium, Nrf2, chlorogenic acid, human intervention study, phase II enzymes",

author = "Nadine Volz and Ute Boettler and Swantje Winkler and Nicole Teller and Christoph Schwarz and Tamara Bakuradze and Gerhard Eisenbrand and Larissa Haupt and Griffiths, \{Lyn R.\} and Herbert Stiebitz and Gerhard Bytof and Ingo Lantz and Roman Lang and Thomas Hofmann and Veronika Somoza and Doris Marko",

year = "2012",

month = sep,

day = "26",

doi = "10.1021/jf302258u",

language = "English",

volume = "60",

pages = "9631--9641",

journal = "Journal of Agricultural and Food Chemistry",

issn = "0021-8561",

publisher = "American Chemical Society",

number = "38",

}

Volz, N, Boettler, U, Winkler, S, Teller, N, Schwarz, C, Bakuradze, T, Eisenbrand, G, Haupt, L, Griffiths, LR, Stiebitz, H, Bytof, G, Lantz, I, Lang, R, Hofmann, T, Somoza, V & Marko, D 2012, 'Effect of coffee combining green coffee bean constituents with typical roasting products on the Nrf2/ARE pathway in vitro and in vivo', Journal of Agricultural and Food Chemistry, vol. 60, no. 38, pp. 9631-9641. https://doi.org/10.1021/jf302258u

Effect of coffee combining green coffee bean constituents with typical roasting products on the Nrf2/ARE pathway in vitro and in vivo. / Volz, Nadine (First Author); Boettler, Ute (Co-Author); Winkler, Swantje (Co-Author) et al.
In: Journal of Agricultural and Food Chemistry, Vol. 60, No. 38, 26.09.2012, p. 9631-9641.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Effect of coffee combining green coffee bean constituents with typical roasting products on the Nrf2/ARE pathway in vitro and in vivo

AU - Volz, Nadine

AU - Boettler, Ute

AU - Winkler, Swantje

AU - Teller, Nicole

AU - Schwarz, Christoph

AU - Bakuradze, Tamara

AU - Eisenbrand, Gerhard

AU - Haupt, Larissa

AU - Griffiths, Lyn R.

AU - Stiebitz, Herbert

AU - Bytof, Gerhard

AU - Lantz, Ingo

AU - Lang, Roman

AU - Hofmann, Thomas

AU - Somoza, Veronika

AU - Marko, Doris

PY - 2012/9/26

Y1 - 2012/9/26

N2 - This study investigated Nrf2-activating properties of a coffee blend combining raw coffee bean constituents with 5-O-caffeoylquinic acid (CGA) as a lead component with typical roasting products such as N-methylpyridinium (NMP). In cell culture (HT29) the respective coffee extract (CN-CE) increased nuclear Nrf2 translocation and enhanced the transcription of ARE-dependent genes as exemplified for NAD(P)H:quinone oxidoreductase and glutathione-S-transferase (GST)A1, reflected in the protein level by an increase in GST enzyme activity. In a pilot human intervention study (29 healthy volunteers), daily consumption of 750 mL of CN-coffee for 4 weeks increased Nrf2 transcription in peripheral blood lymphocytes on average. However, the transcriptional response pattern of Nrf2/ARE-dependent genes showed substantial interindividual variations. The presence of SNPs in the Nrf2-promoter, reported recently, as well as the detection of GSTT1*0 (null) genotypes in the study collective strengthens the hypothesis that coffee acts as a modulator of Nrf2-dependent gene response in humans, but genetic polymorphisms play an important role in the individual response pattern.

AB - This study investigated Nrf2-activating properties of a coffee blend combining raw coffee bean constituents with 5-O-caffeoylquinic acid (CGA) as a lead component with typical roasting products such as N-methylpyridinium (NMP). In cell culture (HT29) the respective coffee extract (CN-CE) increased nuclear Nrf2 translocation and enhanced the transcription of ARE-dependent genes as exemplified for NAD(P)H:quinone oxidoreductase and glutathione-S-transferase (GST)A1, reflected in the protein level by an increase in GST enzyme activity. In a pilot human intervention study (29 healthy volunteers), daily consumption of 750 mL of CN-coffee for 4 weeks increased Nrf2 transcription in peripheral blood lymphocytes on average. However, the transcriptional response pattern of Nrf2/ARE-dependent genes showed substantial interindividual variations. The presence of SNPs in the Nrf2-promoter, reported recently, as well as the detection of GSTT1*0 (null) genotypes in the study collective strengthens the hypothesis that coffee acts as a modulator of Nrf2-dependent gene response in humans, but genetic polymorphisms play an important role in the individual response pattern.

KW - N-methylpyridinium

KW - Nrf2

KW - chlorogenic acid

KW - human intervention study

KW - phase II enzymes

UR - https://www.scopus.com/pages/publications/84866651255

U2 - 10.1021/jf302258u

DO - 10.1021/jf302258u

M3 - Article

C2 - 22946519

AN - SCOPUS:84866651255

SN - 0021-8561

VL - 60

SP - 9631

EP - 9641

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 38

ER -

Effect of coffee combining green coffee bean constituents with typical roasting products on the Nrf2/ARE pathway in vitro and in vivo

Abstract

Keywords

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