Control of Ligand-Binding Specificity Using Photocleavable Linkers in AFM Force Spectroscopy

Melanie Koehler; Cristina Lo Giudice; Philipp Vogl; Andreas Ebner; Peter Hinterdorfer; Hermann J. Gruber; David Alsteens

doi:10.1021/acs.nanolett.0c01426

Control of Ligand-Binding Specificity Using Photocleavable Linkers in AFM Force Spectroscopy

Melanie Koehler (Shared First Author), Cristina Lo Giudice (Shared First Author), Philipp Vogl (Co-Author), Andreas Ebner (Co-Author), Peter Hinterdorfer (Co-Author), Hermann J. Gruber (Co-Author), David Alsteens^* (Last Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

In recent decades, atomic force microscopy (AFM), in particular the force spectroscopy mode, has become a method of choice to study biomolecular interactions at the single-molecule level. However, grafting procedures as well as determining binding specificity remain challenging. We report here an innovative approach based on a photocleavable group that enables in situ release of the ligands bound to the AFM tip and thus allows direct assessment of the binding specificity. Applicable to a wide variety of molecules, the strategy presented here provides new opportunities to study specific interactions and deliver single molecules with high spatiotemporal resolution in a wide range of applications, including AFM-based cell biology.

Original language	English
Pages (from-to)	4038-4042
Number of pages	5
Journal	Nano Letters
Volume	20
Issue number	5
DOIs	https://doi.org/10.1021/acs.nanolett.0c01426
State	Published - 13 May 2020
Externally published	Yes

Keywords

Atomic force microscopy
PEG linker
interaction
optochemical probe
photocleavable
photolysis
single-molecule force spectroscopy

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10.1021/acs.nanolett.0c01426

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title = "Control of Ligand-Binding Specificity Using Photocleavable Linkers in AFM Force Spectroscopy",

abstract = "In recent decades, atomic force microscopy (AFM), in particular the force spectroscopy mode, has become a method of choice to study biomolecular interactions at the single-molecule level. However, grafting procedures as well as determining binding specificity remain challenging. We report here an innovative approach based on a photocleavable group that enables in situ release of the ligands bound to the AFM tip and thus allows direct assessment of the binding specificity. Applicable to a wide variety of molecules, the strategy presented here provides new opportunities to study specific interactions and deliver single molecules with high spatiotemporal resolution in a wide range of applications, including AFM-based cell biology.",

keywords = "Atomic force microscopy, PEG linker, interaction, optochemical probe, photocleavable, photolysis, single-molecule force spectroscopy",

author = "Melanie Koehler and \{Lo Giudice\}, Cristina and Philipp Vogl and Andreas Ebner and Peter Hinterdorfer and Gruber, \{Hermann J.\} and David Alsteens",

note = "Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society.",

year = "2020",

month = may,

day = "13",

doi = "10.1021/acs.nanolett.0c01426",

language = "English",

volume = "20",

pages = "4038--4042",

journal = "Nano Letters",

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TY - JOUR

T1 - Control of Ligand-Binding Specificity Using Photocleavable Linkers in AFM Force Spectroscopy

AU - Koehler, Melanie

AU - Lo Giudice, Cristina

AU - Vogl, Philipp

AU - Ebner, Andreas

AU - Hinterdorfer, Peter

AU - Gruber, Hermann J.

AU - Alsteens, David

PY - 2020/5/13

Y1 - 2020/5/13

N2 - In recent decades, atomic force microscopy (AFM), in particular the force spectroscopy mode, has become a method of choice to study biomolecular interactions at the single-molecule level. However, grafting procedures as well as determining binding specificity remain challenging. We report here an innovative approach based on a photocleavable group that enables in situ release of the ligands bound to the AFM tip and thus allows direct assessment of the binding specificity. Applicable to a wide variety of molecules, the strategy presented here provides new opportunities to study specific interactions and deliver single molecules with high spatiotemporal resolution in a wide range of applications, including AFM-based cell biology.

AB - In recent decades, atomic force microscopy (AFM), in particular the force spectroscopy mode, has become a method of choice to study biomolecular interactions at the single-molecule level. However, grafting procedures as well as determining binding specificity remain challenging. We report here an innovative approach based on a photocleavable group that enables in situ release of the ligands bound to the AFM tip and thus allows direct assessment of the binding specificity. Applicable to a wide variety of molecules, the strategy presented here provides new opportunities to study specific interactions and deliver single molecules with high spatiotemporal resolution in a wide range of applications, including AFM-based cell biology.

KW - Atomic force microscopy

KW - PEG linker

KW - interaction

KW - optochemical probe

KW - photocleavable

KW - photolysis

KW - single-molecule force spectroscopy

UR - https://www.scopus.com/pages/publications/85084695379

U2 - 10.1021/acs.nanolett.0c01426

DO - 10.1021/acs.nanolett.0c01426

M3 - Article

C2 - 32320256

AN - SCOPUS:85084695379

SN - 1530-6984

VL - 20

SP - 4038

EP - 4042

JO - Nano Letters

JF - Nano Letters

IS - 5

ER -

Control of Ligand-Binding Specificity Using Photocleavable Linkers in AFM Force Spectroscopy

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Keywords

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