Comprehensive Quantification of (Poly)phenols in Lotus japonicus with and without Arbuscular Mycorrhizal Symbiosis

Josef L. Ranner; Georg Stabl; Cindy Martyniak; Michael Paries; Andrea Spaccasassi; Caroline Gutjahr; Timo D. Stark; Corinna Dawid

doi:10.1021/acs.jafc.5c02061

Comprehensive Quantification of (Poly)phenols in Lotus japonicus with and without Arbuscular Mycorrhizal Symbiosis

Josef L. Ranner, Georg Stabl, Cindy Martyniak, Michael Paries, Andrea Spaccasassi, Caroline Gutjahr, Timo D. Stark, Corinna Dawid^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

In the present study, a highly specific, accurate, and robust ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous quantification of 50 plant (poly)phenol analytes was developed and validated to assess the effect of arbuscular mycorrhizal (AM) symbiosis on the (poly)phenolic content of the model legume Lotus japonicus (L. japonicus). Determination of molar concentrations of analytes in roots and shoots of wild-type and AM mutant L. japonicus (with and without AM symbiosis, respectively) revealed an overall increase in (poly)phenols in mycorrhizal plants. Time-course observation over 10 weeks showed a shift in (poly)phenol concentrations, especially in the roots. In total, 13 analytes were notably more abundant in young AM roots, suggesting a potential role in symbiosis initiation. An accumulation of various (poly)phenols at later stages of symbiosis might indicate a potential involvement in arbuscule degradation or AM autoregulation.

Original language	English
Pages (from-to)	14044-14057
Number of pages	14
Journal	Journal of Agricultural and Food Chemistry
Volume	73
Issue number	22
DOIs	https://doi.org/10.1021/acs.jafc.5c02061
State	Published - 4 Jun 2025
Externally published	Yes

Keywords

arbuscular mycorrhiza
flavonoids
Lotus japonicus
plant polyphenols
quantification method
symbiosis
UHPLC−MS/MS

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10.1021/acs.jafc.5c02061

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title = "Comprehensive Quantification of (Poly)phenols in Lotus japonicus with and without Arbuscular Mycorrhizal Symbiosis",

abstract = "In the present study, a highly specific, accurate, and robust ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous quantification of 50 plant (poly)phenol analytes was developed and validated to assess the effect of arbuscular mycorrhizal (AM) symbiosis on the (poly)phenolic content of the model legume Lotus japonicus (L. japonicus). Determination of molar concentrations of analytes in roots and shoots of wild-type and AM mutant L. japonicus (with and without AM symbiosis, respectively) revealed an overall increase in (poly)phenols in mycorrhizal plants. Time-course observation over 10 weeks showed a shift in (poly)phenol concentrations, especially in the roots. In total, 13 analytes were notably more abundant in young AM roots, suggesting a potential role in symbiosis initiation. An accumulation of various (poly)phenols at later stages of symbiosis might indicate a potential involvement in arbuscule degradation or AM autoregulation.",

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author = "Ranner, \{Josef L.\} and Georg Stabl and Cindy Martyniak and Michael Paries and Andrea Spaccasassi and Caroline Gutjahr and Stark, \{Timo D.\} and Corinna Dawid",

note = "Publisher Copyright: {\textcopyright} 2025 The Authors. Published by American Chemical Society.",

year = "2025",

month = jun,

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doi = "10.1021/acs.jafc.5c02061",

language = "English",

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T1 - Comprehensive Quantification of (Poly)phenols in Lotus japonicus with and without Arbuscular Mycorrhizal Symbiosis

AU - Ranner, Josef L.

AU - Stabl, Georg

AU - Martyniak, Cindy

AU - Paries, Michael

AU - Spaccasassi, Andrea

AU - Gutjahr, Caroline

AU - Stark, Timo D.

AU - Dawid, Corinna

PY - 2025/6/4

Y1 - 2025/6/4

N2 - In the present study, a highly specific, accurate, and robust ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous quantification of 50 plant (poly)phenol analytes was developed and validated to assess the effect of arbuscular mycorrhizal (AM) symbiosis on the (poly)phenolic content of the model legume Lotus japonicus (L. japonicus). Determination of molar concentrations of analytes in roots and shoots of wild-type and AM mutant L. japonicus (with and without AM symbiosis, respectively) revealed an overall increase in (poly)phenols in mycorrhizal plants. Time-course observation over 10 weeks showed a shift in (poly)phenol concentrations, especially in the roots. In total, 13 analytes were notably more abundant in young AM roots, suggesting a potential role in symbiosis initiation. An accumulation of various (poly)phenols at later stages of symbiosis might indicate a potential involvement in arbuscule degradation or AM autoregulation.

AB - In the present study, a highly specific, accurate, and robust ultrahigh-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method for the simultaneous quantification of 50 plant (poly)phenol analytes was developed and validated to assess the effect of arbuscular mycorrhizal (AM) symbiosis on the (poly)phenolic content of the model legume Lotus japonicus (L. japonicus). Determination of molar concentrations of analytes in roots and shoots of wild-type and AM mutant L. japonicus (with and without AM symbiosis, respectively) revealed an overall increase in (poly)phenols in mycorrhizal plants. Time-course observation over 10 weeks showed a shift in (poly)phenol concentrations, especially in the roots. In total, 13 analytes were notably more abundant in young AM roots, suggesting a potential role in symbiosis initiation. An accumulation of various (poly)phenols at later stages of symbiosis might indicate a potential involvement in arbuscule degradation or AM autoregulation.

KW - arbuscular mycorrhiza

KW - flavonoids

KW - Lotus japonicus

KW - plant polyphenols

KW - quantification method

KW - symbiosis

KW - UHPLC−MS/MS

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DO - 10.1021/acs.jafc.5c02061

M3 - Article

C2 - 40418640

AN - SCOPUS:105006679798

SN - 0021-8561

VL - 73

SP - 14044

EP - 14057

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

IS - 22

ER -

Comprehensive Quantification of (Poly)phenols in Lotus japonicus with and without Arbuscular Mycorrhizal Symbiosis

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Keywords

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