Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography

Jory Lietard; Nicole Kretschy; Matej Sack; Alexander S. Wahba; Mark M. Somoza; Masad J. Damha

doi:10.1039/c4cc05771f

Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography

Jory Lietard, Nicole Kretschy, Matej Sack, Alexander S. Wahba, Mark M. Somoza, Masad J. Damha^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries. We describe the use of LC-MS to analyse DNA and RNA oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using light-directed in situ chemistry. The data yield essential information on array quality and sequence identity.

Original language	English
Pages (from-to)	12903-12906
Number of pages	4
Journal	Chemical Communications
Volume	50
Issue number	85
DOIs	https://doi.org/10.1039/c4cc05771f
State	Published - 30 Sep 2014
Externally published	Yes

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abstract = "Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries. We describe the use of LC-MS to analyse DNA and RNA oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using light-directed in situ chemistry. The data yield essential information on array quality and sequence identity.",

author = "Jory Lietard and Nicole Kretschy and Matej Sack and Wahba, \{Alexander S.\} and Somoza, \{Mark M.\} and Damha, \{Masad J.\}",

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AU - Lietard, Jory

AU - Kretschy, Nicole

AU - Sack, Matej

AU - Wahba, Alexander S.

AU - Somoza, Mark M.

AU - Damha, Masad J.

PY - 2014/9/30

Y1 - 2014/9/30

N2 - Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries. We describe the use of LC-MS to analyse DNA and RNA oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using light-directed in situ chemistry. The data yield essential information on array quality and sequence identity.

AB - Assessing synthesis efficiency, errors, failed deprotections, and chemical and enzymatic degradation of oligonucleotides on microarrays is essential for improving existing in situ synthesis methods, and for the development of new chemistries. We describe the use of LC-MS to analyse DNA and RNA oligonucleotides deprotected and cleaved under basic conditions from microarrays fabricated using light-directed in situ chemistry. The data yield essential information on array quality and sequence identity.

UR - https://www.scopus.com/pages/publications/84907651763

U2 - 10.1039/c4cc05771f

DO - 10.1039/c4cc05771f

M3 - Article

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AN - SCOPUS:84907651763

SN - 1359-7345

VL - 50

SP - 12903

EP - 12906

JO - Chemical Communications

JF - Chemical Communications

IS - 85

ER -

Base-cleavable microarrays for the characterization of DNA and RNA oligonucleotides synthesized in situ by photolithography

Abstract

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