A study of oxygen isotope scrambling in the enzymic and non-enzymic oxidation of linoleic acid

P. Schieberle; W. Grosch; H. Kexel; H. L. Schmidt

doi:10.1016/0005-2760(81)90290-3

A study of oxygen isotope scrambling in the enzymic and non-enzymic oxidation of linoleic acid

P. Schieberle^*
, W. Grosch
, H. Kexel
, H. L. Schmidt

^*Corresponding author for this work

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Lipoxygenases-1 and -2 isolated from soybeans were incubated with linoleic acid in the presence of a mixture of ¹⁶O₂ and ¹⁸O₂. The formation of ¹⁶O/¹⁸O-molecules which is indicative for a head-to-head reaction of peroxy radicals was determined and compared with that produced during autoxidation of a linoleic acid emulsion in the presence of ferric ions. Lipoxygenase-1 was much less active in scrambling than lipoxygenase-2 which was comparable to that found in the autoxidation reaction.

Original language	English
Pages (from-to)	322-326
Number of pages	5
Journal	Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume	666
Issue number	3
DOIs	https://doi.org/10.1016/0005-2760(81)90290-3
State	Published - 23 Dec 1981

Keywords

Autoxidation
Head-to-head condensation
Linoleic acid
Lipoxygenase
Oxygen isotope
Peroxy radical
Scrambling

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10.1016/0005-2760(81)90290-3

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title = "A study of oxygen isotope scrambling in the enzymic and non-enzymic oxidation of linoleic acid",

abstract = "Lipoxygenases-1 and -2 isolated from soybeans were incubated with linoleic acid in the presence of a mixture of 16O2 and 18O2. The formation of 16O/18O-molecules which is indicative for a head-to-head reaction of peroxy radicals was determined and compared with that produced during autoxidation of a linoleic acid emulsion in the presence of ferric ions. Lipoxygenase-1 was much less active in scrambling than lipoxygenase-2 which was comparable to that found in the autoxidation reaction.",

keywords = "Autoxidation, Head-to-head condensation, Linoleic acid, Lipoxygenase, Oxygen isotope, Peroxy radical, Scrambling",

author = "P. Schieberle and W. Grosch and H. Kexel and Schmidt, \{H. L.\}",

year = "1981",

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doi = "10.1016/0005-2760(81)90290-3",

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journal = "Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism",

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T1 - A study of oxygen isotope scrambling in the enzymic and non-enzymic oxidation of linoleic acid

AU - Schieberle, P.

AU - Grosch, W.

AU - Kexel, H.

AU - Schmidt, H. L.

PY - 1981/12/23

Y1 - 1981/12/23

N2 - Lipoxygenases-1 and -2 isolated from soybeans were incubated with linoleic acid in the presence of a mixture of 16O2 and 18O2. The formation of 16O/18O-molecules which is indicative for a head-to-head reaction of peroxy radicals was determined and compared with that produced during autoxidation of a linoleic acid emulsion in the presence of ferric ions. Lipoxygenase-1 was much less active in scrambling than lipoxygenase-2 which was comparable to that found in the autoxidation reaction.

AB - Lipoxygenases-1 and -2 isolated from soybeans were incubated with linoleic acid in the presence of a mixture of 16O2 and 18O2. The formation of 16O/18O-molecules which is indicative for a head-to-head reaction of peroxy radicals was determined and compared with that produced during autoxidation of a linoleic acid emulsion in the presence of ferric ions. Lipoxygenase-1 was much less active in scrambling than lipoxygenase-2 which was comparable to that found in the autoxidation reaction.

KW - Autoxidation

KW - Head-to-head condensation

KW - Linoleic acid

KW - Lipoxygenase

KW - Oxygen isotope

KW - Peroxy radical

KW - Scrambling

UR - https://www.scopus.com/pages/publications/0019887118

U2 - 10.1016/0005-2760(81)90290-3

DO - 10.1016/0005-2760(81)90290-3

M3 - Article

C2 - 6798993

AN - SCOPUS:0019887118

SN - 0005-2760

VL - 666

SP - 322

EP - 326

JO - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism

JF - Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism

IS - 3

ER -

A study of oxygen isotope scrambling in the enzymic and non-enzymic oxidation of linoleic acid

Abstract

Keywords

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