Description
Unlike standard solid phase synthesis, photolithographic synthesis of DNA uses only moderately acidic reagents, and therefore it is intrinsically less susceptible to fragmentation due to depurination. Nevertheless, synthesis of long oligonucleotides via photolithography has demonstrated lower average yield. Our findings demonstrate chain cleavage of DNA sequences due to guanine depurination and we present methods to optimize synthesis conditions for suppressing depurination thereby resulting in enhanced sequence fidelity and synthesis yield.| Period | 3 Sep 2024 → 6 Sep 2024 |
|---|---|
| Event title | XXV International Round Table on Nucleosides, Nucleotides and Nucleic Acids |
| Event type | Conference |
| Location | Tokyo, JapanShow on map |
Related content
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Research output
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An Efficient Guanine O6 Phosphitylation Repair Strategy for High-Quality DNA Library Synthesis via Digital Nucleic Acid Photolithography
Research output: Working paper › Preprint
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Efficiency of Digital Photolithographic Synthesis of Large, High-Quality DNA Libraries and Microarrays using a Guanine O6 Dephosphitylation Strategy
Research output: Contribution to journal › Article › peer-review