Large-scale photolithographic synthesis for dense information storage in DNA libraries generated from mixed base and trimer phosphoramidites

Activity: Talk or event contributionPoster presentation

Description

Synthetic DNA is a promising medium for routine long-term data storage. Among various synthesis methods, large-scale
photolithographic DNA synthesis, which adapts Caruthers and Beaucage's phosphoramidite chemistry for in situ surface
synthesis, offers the best scalability and cost efficiency for producing large DNA libraries.
To enhance data density beyond the standard 2 bits per coupling, trimer and composite DNA letters are used as sequence
building blocks. Composite DNA letters are created by mixing 2 to 4 DNA bases in specific ratios, which effectively expands
the DNA alphabet. These unique ratios can be identified through sequencing. Additionally, the use of trimer
phosphoramidites introduces new strategies to further improve DNA-based data storage.
Period6 Sep 2024
Event titleXXV International Round Table on Nucleosides: Nucleotides and Nucleic Acids at Katsushika Campus
Event typeConference
Conference number25
LocationTokyo, JapanShow on map
Degree of RecognitionInternational